Ĭhang CP, Hsu CC (2006) The formation and water content of synthetic fiber growing media. Ĭhan YY, Kim KH, Cheah SH (2011) Inhibitory effects of Sargassum polycystum on tyrosinase activity and melanin formation in B16F10 murine melanoma cells. Orthop Clin North Am 18:323–334Ĭaplin JD, Granados NG, James MR et al (2015) Microfluidic organ-on-a-chip technology for advancement of drug development and toxicology. īucholz RW, Carlton A, Holmes RE (1987) Hydroxyapatite and tricalcium phosphate bone graft substitutes. Elsevier, Amsterdamīružauskaitė I, Bironaitė D, Bagdonas E, Bernotienė E (2016) Scaffolds and cells for tissue regeneration: different scaffold pore sizes-different cell effects. īosworth L, Downes S (2011) Electrospinning for tissue regeneration. Woodhead Publishing, Cambridge, pp 345–365īoateng JS, Matthews KH, Stevens HNE, Eccleston GM (2008) Wound healing dressings and drug delivery systems: a review. In: Deng Y, Kuiper J (eds) Functional 3D tissue engineering scaffolds. 3D functional scaffolds for skin tissue engineering. J Biomed Mater Res 58:467–477īhardwaj N, Chouhan D, Mandal BB (2018) 14. J Biomater Sci Polym Ed 13:447–462īélanger MC, Marois Y (2001) Hemocompatibility, biocompatibility, inflammatory and in vivo studies of primary reference materials low-density polyethylene and polydimethylsiloxane: a review. Īucoin L, Griffith CM, Pleizier G et al (2002) Interactions of corneal epithelial cells and surfaces modified with cell adhesion peptide combinations. In addition, the fabricated PDMS scaffolds may also be exploited for a plethora of other applications in tissue engineering and drug delivery.Īnnabi N, Nichol JW, Zhong X et al (2010) Controlling the porosity and microarchitecture of hydrogels for tissue engineering. Thus, the outcomes suggest that PDMS based macroporous scaffold can be used as a potential candidate for skin dressing material. Therefore, scaffolds facilitate a high degree of cellular adhesion while providing a microenvironment necessary for optimal cellular infiltration and viability. Experimental results demonstrate not only a high proliferative potential of the skin tissue-specific cells within the fabricated PDMS based scaffolds but also confirm the presence of several other essential attributes such as high interconnectivity, optimum porosity, excellent mechanical strength, gaseous permeability, promising cell compatibility, water absorption capability and desired surface wettability. In addition, scaffolds were pre-coated with collagen and investigated as a podium for culturing the chosen cells (fibroblast and melanocyte cells). Effective pore sizes of both scaffolds were observed to lie in the desirable range of 152–165 μm. To determine a suitable ratio of salt to PDMS pre-polymer in the scaffold, two different samples with ratios 2:1 and 3:1, were fabricated. In vitro co-culture assessment of epidermal-origin mouse B16-F10 melanocyte cells and mouse L929 fibroblast cells in three-dimensional polymeric scaffolds has been carried out towards developing bio-stable, interconnected, highly macroporous, PDMS based tissue-engineered scaffolds, using the salt leaching method. The lamination of each PDMS layer resulted in a construct with three-directionally interconnected micropores (600圆00 [μm in section) as a PDMS body.In the present study, we propose a platform for topical wound dressing material using a polydimethylsiloxane (PDMS) scaffold in order to enhance the skin healing process. We used a combination of casting mold and lamination process to fabricate PDMS scaffold with controlled pore configuration. The three-dimensional scaffold was designed to investigate the effect of mechanical stress to the cells by utilizing the intrinsic elasticity of the PDMS. The lamination of each PDMS layer resulted in a construct with three-directionally interconnected micropores (600圆00 [μm in section) as a PDMS body.ĪB - In this study, we tried to create a polydimethyl -siloxane (PDMS) scaffold with controlled pore configurations to investigate the proliferation and differentiation of the cells seeded 3-dimensionally. N2 - In this study, we tried to create a polydimethyl -siloxane (PDMS) scaffold with controlled pore configurations to investigate the proliferation and differentiation of the cells seeded 3-dimensionally. T1 - Fabrication of PDMS scaffold with controlled configurations
0 Comments
Leave a Reply. |